General OVA-Ovalbumin ELISA Kit
Overview of OVA-Ovalbumin ELISA Kit
Product name: | OVA-Ovalbumin ELISA Kit |
Reactivity: | General |
Alternative Names: | Ovalbumin |
Assay Type: | Competitive Inhibition |
Sensitivity: | 7 pg/mL |
Standard: | 10000 ng/mL |
Detection Range: | 156.25-10000 pg/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 2 h |
Research Area: | Infection immunity;Immune molecule;Hematology; |
Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with OVA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to OVA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of OVA in the samples is then determined by comparing the OD of the samples to the standard curve. |
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One Step SYBR Green RT-PCR Mix
Additional information
Size | 48T, 96T |
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