General T4-Thyroxine ELISA Kit
General T4-Thyroxine ELISA Kit
€262.00 – €374.00
Specification 96T, 48T
Overview of T4-Thyroxine ELISA Kit
| Product name: | T4-Thyroxine ELISA Kit |
| Reactivity: | General |
| Alternative Names: | 3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 1.42 ng/mL |
| Standard: | 300 ng/mL |
| Detection Range: | 4.69-300 ng/mL |
| Sample Type: | serum, plasma and other biological fluids |
| Assay Length: | 2 h |
| Research Area: | Endocrinology;Hormone metabolism; |
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with T4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
One Step SYBR Green RT-PCR Mix
Additional information
| Size | 48T, 96T |
|---|
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