Name: General T4-Thyroxine ELISA Kit - Gencyst
SKU: ELK1204
Availability: InStock

General T4-Thyroxine ELISA Kit

Price range: €262.00 through €374.00

Specification 96T, 48T

SKU: ELK1204 Category: ELISA Kit Tag: ELISA Kit

Overview of T4-Thyroxine ELISA Kit

Product name:	T4-Thyroxine ELISA Kit
Reactivity:	General
Alternative Names:	3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine
Assay Type:	Competitive Inhibition
Sensitivity:	1.42 ng/mL
Standard:	300 ng/mL
Detection Range:	4.69-300 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	2 h
Research Area:	Endocrinology;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with T4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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