Name: Human ALPI-Alkaline Phosphatase, Intestinal ELISA Kit - Gencyst
SKU: ELK1758
Availability: InStock

Human ALPI-Alkaline Phosphatase, Intestinal ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK1758 Category: ELISA Kit Tag: ELISA Kit

Overview of Human ALPI-Alkaline Phosphatase, Intestinal ELISA Kit

Product name:	Human ALPI-Alkaline Phosphatase, Intestinal ELISA Kit
Reactivity:	Human
Alternative Names:	CIP; IAP; Calf Intestinal Alkaline Phosphatase
Assay Type:	Sandwich
Sensitivity:	0.55 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5 h
Research Area:	Enzyme & Kinase;Metabolic pathway;Gastroenterology;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ALPI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALPI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ALPI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ALPI in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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