Name: Human AST-Aspartate Aminotransferase ELISA Kit - Gencyst
SKU: ELK1966
Availability: InStock

Human AST-Aspartate Aminotransferase ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK1966 Category: ELISA Kit Tag: ELISA Kit

Overview of Human AST-Aspartate Aminotransferase ELISA Kit

Product name:	Human AST-Aspartate Aminotransferase ELISA Kit
Reactivity:	Human
Alternative Names:	cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble
Assay Type:	Sandwich
Sensitivity:	0.119 U/L
Standard:	20 U/L
Detection Range:	0.32-20 U/L
Sample Type:	serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5 h
Research Area:	Enzyme & Kinase;Tumor immunity;Infection immunity;Hepatology;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AST in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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