Name: Human E-Cad-E-Cadherin ELISA Kit - Gencyst
SKU: ELK1006
Availability: InStock

Human E-Cad-E-Cadherin ELISA Kit

Price range: €262.00 through €364.00

Specification 96T, 48T

SKU: ELK1006 Category: ELISA Kit Tag: ELISA Kit

Overview of Human E-Cad-E-Cadherin ELISA Kit

Product name:	Human E-Cad-E-Cadherin ELISA Kit
Reactivity:	Human
Alternative Names:	CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial
Assay Type:	Sandwich
Sensitivity:	25.1 pg/mL
Standard:	4000 pg/mL
Detection Range:	62.5-4000 pg/mL
Sample Type:	serum, plasma, urine, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	CD & Adhesion molecule;Tumor immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.