Human CDKN2A-Cyclin Dependent Kinase Inhibitor 2A ELISA Kit
Human CDKN2A-Cyclin Dependent Kinase Inhibitor 2A ELISA Kit
€318.00 – €455.00
Specification 96T, 48T
Overview of Human CDKN2A-Cyclin Dependent Kinase Inhibitor 2A ELISA Kit
| Product name: | Human CDKN2A-Cyclin Dependent Kinase Inhibitor 2A ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | P16; ARF; CDK4I; CMM2; INK4; INK4a; MLM; MTS1; TP16; P14; P14ARF; P16INK4a; P19; Melanoma P16; Cyclin-dependent kinase 4 inhibitor A; Multiple tumor suppressor 1 |
| Assay Type: | Sandwich |
| Sensitivity: | 0.262 ng/mL |
| Standard: | 40 ng/mL |
| Detection Range: | 0.63-40 ng/mL |
| Sample Type: | tissue homogenates, cell lysates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Tumor immunity;Developmental science; |
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CDKN2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CDKN2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CDKN2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CDKN2A in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
| Size | 48T, 96T |
|---|
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