Name: Human CYP7A1-Cytochrome P450 7A1 ELISA Kit - Gencyst
SKU: ELK2460
Availability: InStock

Human CYP7A1-Cytochrome P450 7A1 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK2460 Category: ELISA Kit Tag: ELISA Kit

Overview of Human CYP7A1-Cytochrome P450 7A1 ELISA Kit

Product name:	Human CYP7A1-Cytochrome P450 7A1 ELISA Kit
Reactivity:	Human
Alternative Names:	CYP7; CYP7-A1; CYPVII; Cholesterol 7 Alpha Hydroxylase; Cytochrome P450 Family 7 Subfamily A Polypeptide 1
Assay Type:	Sandwich
Sensitivity:	0.31 ng/mL
Standard:	50 ng/mL
Detection Range:	0.79-50 ng/mL
Sample Type:	tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5 h
Research Area:	Metabolic pathway;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYP7A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYP7A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYP7A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYP7A1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T