Name: Human GRa-Glucocorticoid Receptor Alpha ELISA Kit - Gencyst
SKU: ELK1666

Human GRa-Glucocorticoid Receptor Alpha ELISA Kit

Specification 96T, 48T

SKU: ELK1666 Category: ELISA Kit Tag: ELISA Kit

Overview of Human GRa-Glucocorticoid Receptor Alpha ELISA Kit

Product name:	Human GRa-Glucocorticoid Receptor Alpha ELISA Kit
Reactivity:	Human
Alternative Names:	GR-A
Assay Type:	Sandwich
Sensitivity:	0.118 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5 h
Research Area:	Signal transduction;Metabolic pathway;Endocrinology;Hormone metabolism;
	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GRa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GRa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GRa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GRa in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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