Name: Human HSP90b1-Heat Shock Protein 90kDa Beta 1 ELISA Kit - Gencyst
SKU: ELK1609
Availability: InStock

Human HSP90b1-Heat Shock Protein 90kDa Beta 1 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK1609 Category: ELISA Kit Tag: ELISA Kit

Overview of Human HSP90b1-Heat Shock Protein 90kDa Beta 1 ELISA Kit

Product name:	Human HSP90b1-Heat Shock Protein 90kDa Beta 1 ELISA Kit
Reactivity:	Human
Alternative Names:	HSP90-B1; ECGP; GP96; GRP94; TRA1; Glycoprotein 96; Tumor Rejection Antigen(gp96)1; 94 kDa glucose-regulated protein; Endoplasmin
Assay Type:	Sandwich
Sensitivity:	0.127 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5 h
Research Area:	Signal transduction;Immune molecule;Developmental science;
	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSP90b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSP90b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSP90b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSP90b1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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