Name: Human IGFBP4-Insulin Like Growth Factor Binding Protein 4 ELISA Kit - Gencyst
SKU: ELK1141
Availability: InStock

Human IGFBP4-Insulin Like Growth Factor Binding Protein 4 ELISA Kit

€262.00 – €364.00

Specification 96T, 48T

Size

SKU: ELK1141 Category: ELISA Kit Tag: ELISA Kit

Overview of Human IGFBP4-Insulin Like Growth Factor Binding Protein 4 ELISA Kit

Product name:	Human IGFBP4-Insulin Like Growth Factor Binding Protein 4 ELISA Kit
Reactivity:	Human
Alternative Names:	BP4; HT29-IGFBP; IBP4
Assay Type:	Sandwich
Sensitivity:	0.129 ng/mL
Standard:	20 pg/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5 h
Research Area:	Apoptosis;Tumor immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IGFBP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IGFBP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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