Human KLF4-Kruppel Like Factor 4, Gut ELISA Kit

Human KLF4-Kruppel Like Factor 4, Gut ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK1824 Category: Tag:

Overview of Human KLF4-Kruppel Like Factor 4, Gut ELISA Kit

Product name:

Human KLF4-Kruppel Like Factor 4, Gut ELISA Kit

Reactivity: Human
Alternative Names: EZF; GKLF; Gut-Enriched KrĂĽppel-Like Factor; Epithelial zinc finger protein EZF
Assay Type: Sandwich
Sensitivity: 0.058 ng/mL
Standard: 10 pg/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5 h
Research Area: Signal transduction;Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KLF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KLF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLF4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

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