Human LAB7-2-B-Lymphocyte Activation Antigen B7-2 ELISA Kit
Human LAB7-2-B-Lymphocyte Activation Antigen B7-2 ELISA Kit
€318.00 – €455.00
Specification 96T, 48T
Overview of Human LAB7-2-B-Lymphocyte Activation Antigen B7-2 ELISA Kit
Product name: | Human LAB7-2-B-Lymphocyte Activation Antigen B7-2 ELISA Kit |
Reactivity: | Human |
Alternative Names: | CD86; B7-2; B70; FUN-1; CD28LG2; CD28 Antigen Ligand 2,B7-2 Antigen; CTLA-4 counter-receptor B7.2 |
Assay Type: | Sandwich |
Sensitivity: | 0.274 ng/mL |
Standard: | 10 ng/mL |
Detection Range: | 0.16-10 ng/mL |
Sample Type: | Tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule; |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAB7-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAB7-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAB7-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAB7-2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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