Name: Human ME20M-Melanoma Associated ME20 ELISA Kit - Gencyst
SKU: ELK1886
Availability: InStock

Human ME20M-Melanoma Associated ME20 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK1886 Category: ELISA Kit Tag: ELISA Kit

Overview of Human ME20M-Melanoma Associated ME20 ELISA Kit

Product name:	Human ME20M-Melanoma Associated ME20 ELISA Kit
Reactivity:	Human
Alternative Names:	PMEL; GP100; SI; SIL; SILV; Pmel17; Silver Homolog; Premelanosome Protein; Silver Locus Protein Homolog; 95 kDa melanocyte-specific secreted glycoprotein
Assay Type:	Sandwich
Sensitivity:	0.057 ng/mL
Standard:	10 pg/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5 h
Research Area:	Enzyme & Kinase;Tumor immunity;Dermatology;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ME20M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ME20M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ME20M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ME20M in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T, 96T,48T

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