Human PAFAH2-Platelet Activating Factor Acetylhydrolase 2 ELISA Kit

Human PAFAH2-Platelet Activating Factor Acetylhydrolase 2 ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK1780 Category: Tag:

Overview of Human PAFAH2-Platelet Activating Factor Acetylhydrolase 2 ELISA Kit

Product name: Human PAFAH2-Platelet Activating Factor Acetylhydrolase 2 ELISA Kit
Reactivity: Human
Alternative Names: SD-PLA2; Serine-dependent phospholipase A2; Platelet-activating factor acetylhydrolase 2, cytoplasmic
Assay Type: Sandwich
Sensitivity: 0.105 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Plasma.
Assay Length: 3.5 h
Research Area: Enzyme & Kinase;Metabolic pathway;Hematology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAFAH2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAFAH2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAFAH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAFAH2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

Reviews

There are no reviews yet.

Be the first to review “Human PAFAH2-Platelet Activating Factor Acetylhydrolase 2 ELISA Kit”

Your email address will not be published. Required fields are marked *

Filter by price