Human Tie2-TEK Tyrosine Kinase, Endothelial ELISA Kit

Human Tie2-TEK Tyrosine Kinase, Endothelial ELISA Kit

262.00374.00

Specification 96T, 48T

SKU: ELK1188 Category: Tag:

Overview of Human Tie2-TEK Tyrosine Kinase, Endothelial ELISA Kit

Product name: Human Tie2-TEK Tyrosine Kinase, Endothelial ELISA Kit
Reactivity: Human
Alternative Names: CD202b; CD202-B; TEK; VMCM1; Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains 2; Venous Malformations Multiple Cutaneous And Mucosal
Assay Type: Sandwich
Sensitivity: 0.37 ng/mL
Standard: 60 pg/mL
Detection Range: 0.94-60 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5 h
Research Area: Enzyme & Kinase;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Tie2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Tie2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Tie2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Tie2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

 

Additional information

Size

48T, 96T

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