Name: Human VTN-Vitronectin ELISA Kit - Gencyst
SKU: ELK1744
Availability: InStock

Human VTN-Vitronectin ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK1744 Category: ELISA Kit Tag: ELISA Kit

Overview of Human VTN-Vitronectin ELISA Kit

Product name:	Human VTN-Vitronectin ELISA Kit
Reactivity:	Human
Alternative Names:	V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein
Assay Type:	Sandwich
Sensitivity:	2.74 ng/mL
Standard:	400 ng/mL
Detection Range:	6.25-400 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Signal transduction;Metabolic pathway;Tumor immunity;Hepatology;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VTN in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T, 96T,48T

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