Mouse DKK1-Dickkopf Related Protein 1 ELISA Kit
Mouse DKK1-Dickkopf Related Protein 1 ELISA Kit
€262.00 – €364.00
Specification 96T, 48T
Overview of Mouse DKK1-Dickkopf Related Protein 1 ELISA Kit
| Product name: | Mouse DKK1-Dickkopf Related Protein 1 ELISA Kit |
| Reactivity: | Mouse |
| Alternative Names: | SK; Dickkopf Homolog 1(Xenopus Laevis) |
| Assay Type: | Sandwich |
| Sensitivity: | 0.125 ng/mL |
| Standard: | 20 ng/mL |
| Detection Range: | 0.32-20 ng/mL |
| Sample Type: | Serum, plasma and other biological fluids. |
| Assay Length: | 3.5 h |
| Research Area: | Signal transduction;Tumor immunity;Hematology;Developmental science;Bone metabolism; |
| Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DKK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DKK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DKK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DKK1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
| Size | 48T, 96T |
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