Overview of Mouse Dyn-Big Dynorphin ELISA Kit
Product name: |
Mouse Dyn-Big Dynorphin ELISA Kit |
Reactivity: |
Mouse |
Alternative Names: |
B-DYN; Biocytin 13 Dynorphin A 1-13 Amide |
Assay Type: |
Competitive Inhibition |
Sensitivity: |
1.35 pg/mL |
Standard: |
300 pg/mL |
Detection Range: |
4.69-300 pg/mL |
Sample Type: |
Serum, plasma and other biological fluids. |
Assay Length: |
2 h |
Research Area: |
Signal transduction;Neuro science; |
Test Principle: |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse Dyn protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Dyn. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Dyn in the samples is then determined by comparing the OD of the samples to the standard curve. |
dNTP Mix PCR Grade 10 mM each
Pfu DNA Polymerase
One Step SYBR Green RT-PCR Mix
Hot start Taq DNA Polymerase
EntiLink PCR Master Mix