Mouse tPA-Plasminogen Activator, Tissue ELISA Kit
Mouse tPA-Plasminogen Activator, Tissue ELISA Kit
Specification 96T, 48T
Overview of Mouse tPA-Plasminogen Activator, Tissue ELISA Kit
| Product name: | Mouse tPA-Plasminogen Activator, Tissue ELISA Kit |
| Reactivity: | Mouse |
| Alternative Names: | PLAT; T-PA; t-plasminogen activator; Alteplase; Reteplase |
| Assay Type: | Sandwich |
| Sensitivity: | 0.067 ng/mL |
| Standard: | 10 pg/mL |
| Detection Range: | 0.16-10 ng/mL |
| Sample Type: | serum, plasma and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Metabolic pathway;Hematology; |
| Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse tPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse tPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse tPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse tPA in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
| Size | 96T,48T |
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