Name: Rabbit OPN-Osteopontin ELISA Kit - Gencyst
SKU: ELK1541
Availability: InStock

Rabbit OPN-Osteopontin ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK1541 Category: ELISA Kit Tag: ELISA Kit

Overview of Rabbit OPN-Osteopontin ELISA Kit

Product name:	Rabbit OPN-Osteopontin ELISA Kit
Reactivity:	Rabbit
Alternative Names:	SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin
Assay Type:	Sandwich
Sensitivity:	0.064 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5 h
Research Area:	Tumor immunity;Bone metabolism;
	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit OPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit OPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit OPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit OPN in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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