Rat MMP10-Matrix Metalloproteinase 10 ELISA Kit

Rat MMP10-Matrix Metalloproteinase 10 ELISA Kit

Specification 96T, 48T

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SKU: ELK2540

Overview of Rat MMP10-Matrix Metalloproteinase 10 ELISA Kit

Product name: Rat MMP10-Matrix Metalloproteinase 10 ELISA Kit
Reactivity: Rat
Alternative Names: STMY2; SL2; Stromelysin 2; Transin-2
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
Standard: 10 pg/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5 h
Research Area: Tumor immunity;Infection immunity;Cardiovascular biology;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MMP10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MMP10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MMP10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MMP10 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Size

96T,48T

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