Rat TPO-Thyroid Peroxidase ELISA Kit

Rat TPO-Thyroid Peroxidase ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2516 Category: Tag:

Overview of Rat TPO-Thyroid Peroxidase ELISA Kit

Product name: Rat TPO-Thyroid Peroxidase ELISA Kit
Reactivity: Rat
Alternative Names: TPX; thyroperoxidase
Assay Type: Sandwich
Sensitivity: 11.9 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5 h
Research Area: Hematology;Hormone metabolism;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TPO in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

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Additional information

Size

48T, 96T

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