Name: Rat Trx-Thioredoxin ELISA Kit - Gencyst
SKU: ELK1998
Availability: InStock

Rat Trx-Thioredoxin ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK1998 Category: ELISA Kit Tag: ELISA Kit

Overview of RatTrx-Thioredoxin ELISA Kit

Product name:	Rat Trx-Thioredoxin ELISA Kit
Reactivity:	Rat
Alternative Names:	TXN; ADF; SASP; TRDX; TRX1; ATL-derived factor; Surface-associated sulphydryl protein
Assay Type:	Sandwich
Sensitivity:	0.115 ng/mL
Standard:	20 pg/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5 h
Research Area:	Metabolic pathway;Developmental science;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Trx. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Trx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Trx, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Trx in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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