Rat IGFBP2-Insulin Like Growth Factor Binding Protein 2 ELISA Kit
Rat IGFBP2-Insulin Like Growth Factor Binding Protein 2 ELISA Kit
€318.00 – €455.00
Specification 96T, 48T
Overview of Rat IGFBP2-Insulin Like Growth Factor Binding Protein 2 ELISA Kit
| Product name: | Rat IGFBP2-Insulin Like Growth Factor Binding Protein 2 ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | IBP2; IGF-BP53 |
| Assay Type: | Sandwich |
| Sensitivity: | 1.15 ng/mL |
| Standard: | 200 pg/mL |
| Detection Range: | 3.13-200 ng/mL |
| Sample Type: | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Tumor immunity;Endocrinology; |
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IGFBP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IGFBP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IGFBP2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
| Size | 48T, 96T |
|---|
Overview
Rat IGFBP2-Insulin Like Growth Factor Binding Protein 2 ELISA Kit
Alternative Names (Others Name):
AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor
Type of Assay:
Sandwich
Sensitivity (Percision):
5.8 pg/mL
Standard:
1000 pg/mL
Range:
15.63-1000 pg/mL
Type of Sample:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Length of Assay:
3.5h
Area of Research:
Cytokine;Tumor immunity;
Reactivity:
Mouse
Publications
 Date：2021-12-28  
 IF：4.799Linearity
Matrix |
1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-92% | 85-96% | 93-102% | 95-104% |
| EDTA plasma(n=5) | 86-97% | 91-102% | 81-97% | 93-107% |
| Heparin plasma(n=5) | 82-94% | 83-96% | 91-103% | 82-99% |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AREG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Recovery
| Matrix | Recovery range | Average |
| serum(n=5) | 93-101% | 97% |
| EDTA plasma(n=5) | 85-103% | 94% |
| Heparin plasma(n=5) | 95-105% | 100% |
Matrices listed below were spiked with certain level of recombinant AREG and the recovery rates were calculated by comparing the measured value to the expected amount of AREG in samples.
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Standard curve
Concentration (ng/mL) |
OD |
Corrected OD |
| 1000.00 | 2.154 | 2.046 |
| 500.00 | 1.647 | 1.539 |
| 250.00 | 1.157 | 1.049 |
| 125.00 | 0.726 | 0.618 |
| 62.50 | 0.514 | 0.406 |
| 31.25 | 0.318 | 0.210 |
| 15.63 | 0.216 | 0.108 |
| 0.00 | 0.108 | 0.000 |
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