Overview of Human E-Cad-E-Cadherin ELISA Kit
Product name: |
Human E-Cad-E-Cadherin ELISA Kit |
Reactivity: |
Human |
Alternative Names: |
CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial |
Assay Type: |
Sandwich |
Sensitivity: |
25.1 pg/mL |
Standard: |
4000 pg/mL |
Detection Range: |
62.5-4000 pg/mL |
Sample Type: |
serum, plasma, urine, tissue homogenates and other biological fluids |
Assay Length: |
3.5h |
Research Area: |
CD & Adhesion molecule;Tumor immunity; |
Test principle: |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve. |
dNTP Mix PCR Grade 10 mM each
Pfu DNA Polymerase
One Step SYBR Green RT-PCR Mix
Hot start Taq DNA Polymerase
EntiLink PCR Master Mix