Human MIP3a-Macrophage Inflammatory Protein 3 Alpha ELISA Kit
Human MIP3a-Macrophage Inflammatory Protein 3 Alpha ELISA Kit
€262.00 – €364.00
Specification 96T, 48T
Overview of Human MIP3a-Macrophage Inflammatory Protein 3 Alpha ELISA Kit
Product name: | Human MIP3a-Macrophage Inflammatory Protein 3 Alpha ELISA Kit |
Reactivity: | Human |
Alternative Names: | MIP3 Alpha; CCL20; CKb4; LARC; MIP3-A; SCYA20; ST38; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 20; Beta-Chemokine Exodus-1; Liver And Activation-Regulated Chemokine |
Assay Type: | Sandwich |
Sensitivity: | 2.9 pg/mL |
Standard: | 500 pg/mL |
Detection Range: | 7.82-500 pg/mL |
Sample Type: | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Cytokine;Infection immunity; |
Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MIP3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MIP3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MIP3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MIP3a in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T, 96T,48T |
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