Name: Human LEP-Leptin ELISA Kit - Gencyst
SKU: ELK1160
Availability: InStock

Human LEP-Leptin ELISA Kit

€262.00 – €374.00

Specification 96T, 48T

SKU: ELK1160 Category: ELISA Kit Tag: ELISA Kit

Overview of Human LEP-Leptin ELISA Kit

Product name:	Human LEP-Leptin ELISA Kit
Reactivity:	Human
Alternative Names:	OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein
Assay Type:	Sandwich
Sensitivity:	0.058 ng/mL
Standard:	10 pg/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Metabolic pathway;Endocrinology;Cardiovascular biology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LEP in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T