Human ALCAM-Activated Leukocyte Cell Adhesion Molecule ELISA Kit
Human ALCAM-Activated Leukocyte Cell Adhesion Molecule ELISA Kit
€318.00 – €455.00
Specification 96T, 48T
Overview of Human ALCAM-Activated Leukocyte Cell Adhesion Molecule ELISA Kit
Product name: | Human ALCAM-Activated Leukocyte Cell Adhesion Molecule ELISA Kit |
Reactivity: | Human |
Alternative Names: | CD166; MEMD; Activated Leucocyte Cell Adhesion Molecule |
Assay Type: | Sandwich |
Sensitivity: | 0.068 ng/mL |
Standard: | 10 ng/mL |
Detection Range: | 0.16-10 ng/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | CD & Adhesion molecule;Tumor immunity; |
Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ALCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ALCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ALCAM in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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