Overview of Mouse F2-Coagulation Factor II ELISA Kit
Product name: |
Mouse F2-Coagulation Factor II ELISA Kit |
Reactivity: |
Mouse |
Alternative Names: |
FII; TM; PT; Thrombin; Prothrombin; Pro-Thrombin; Activation peptide fragment 1; Activation peptide fragment 2 |
Assay Type: |
Sandwich |
Sensitivity: |
0.59 ng/mL |
Standard: |
100 ng/mL |
Detection Range: |
1.57-100 ng/mL |
Sample Type: |
Plasma. |
Assay Length: |
3.5 h |
Research Area: |
Signal transduction;Hematology; |
|
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse F2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse F2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse F2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
dNTP Mix PCR Grade 10 mM each
Pfu DNA Polymerase
One Step SYBR Green RT-PCR Mix
Hot start Taq DNA Polymerase
EntiLink PCR Master Mix
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