Mouse PIK3C2a-Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide ELISA Kit

Mouse PIK3C2a-Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK1551 Category: Tag:

Overview of Mouse PIK3C2a-Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide ELISA Kit

Product name: Mouse PIK3C2a-Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide ELISA Kit
Reactivity: Mouse
Alternative Names: PI3K2a; CPK; PI3-K-C2(Alpha); PI3-K-C2A; PI3K2-a; Phosphoinositide 3-Kinase 2a; Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
Assay Type: Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5 h
Research Area: Signal transduction;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PIK3C2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIK3C2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PIK3C2a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIK3C2a in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

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