Human PYGL-Glycogen Phosphorylase, Liver ELISA Kit

Human PYGL-Glycogen Phosphorylase, Liver ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK1577 Category: Tag:

Overview of Human PYGL-Glycogen Phosphorylase, Liver ELISA Kit

Product name: Human PYGL-Glycogen Phosphorylase, Liver ELISA Kit
Reactivity: Human
Alternative Names: GPLL; GPBB; Hers Disease; Glycogen Storage Disease Type VI
Assay Type: Sandwich
Sensitivity: 0.115 ng/mL
Standard: 10 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5 h
Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PYGL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PYGL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PYGL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PYGL in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

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