Rat IAPP-Islet Amyloid Polypeptide ELISA Kit
Rat IAPP-Islet Amyloid Polypeptide ELISA Kit
Price range: €318.00 through €455.00
Specification 96T, 48T
Overview of Rat IAPP-Islet Amyloid Polypeptide ELISA Kit
Product name: | Rat IAPP-Islet Amyloid Polypeptide ELISA Kit |
Reactivity: | Rat |
Alternative Names: | IAP; DAP; Amylin; Insulinoma Amyloid Polypeptide; Diabetes-associated peptide |
Assay Type: | Competitive Inhibition |
Sensitivity: | 2.83 pg/mL |
Standard: | 500 ng/mL |
Detection Range: | 7.82-500 pg/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 2 h |
Research Area: | Metabolic pathway;Endocrinology;Hepatology; |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat IAPP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IAPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IAPP in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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