Mouse PPAR-γ-Peroxisome Proliferator Activated Receptor Gamma ELISA Kit
Mouse PPAR-γ-Peroxisome Proliferator Activated Receptor Gamma ELISA Kit
Price range: €318.00 through €455.00
Specification 96T, 48T
Overview of Mouse PPAR-γ-Peroxisome Proliferator Activated Receptor Gamma ELISA Kit
| Product name: | Mouse PPAR-γ-Peroxisome Proliferator Activated Receptor Gamma ELISA Kit |
| Reactivity: | Mouse |
| Alternative Names: | PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3; PPARg; Peroxisome Proliferator Activated Receptor Gamma |
| Assay Type: | Sandwich |
| Sensitivity: | 0.062 ng/mL |
| Standard: | 10 ng/mL |
| Detection Range: | 0.16-10 ng/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Metabolic pathway;Endocrinology;Cardiovascular biology;Developmental science;Bone metabolism; |
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PPAR-γ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PPAR-γ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PPAR-γ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PPAR-γ in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
| Size | 48T, 96T, 96T,48T |
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