Name: Human CEBPa-CCAAT/Enhancer Binding Protein Alpha ELISA Kit - Gencyst
SKU: ELK1850
Availability: InStock

Human CEBPa-CCAAT/Enhancer Binding Protein Alpha ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK1850 Category: ELISA Kit Tag: ELISA Kit

Overview of Human CEBPa-CCAAT/Enhancer Binding Protein Alpha ELISA Kit

Product name:	Human CEBPa-CCAAT/Enhancer Binding Protein Alpha ELISA Kit
Reactivity:	Human
Alternative Names:	CEBP-A
Assay Type:	Sandwich
Sensitivity:	0.055 ng/mL
Standard:	10 pg/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids.
Assay Length:	3.5 h
Research Area:	Signal transduction;Metabolic pathway;Cardiovascular biology;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CEBPa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CEBPa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CEBPa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CEBPa in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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