Human EPHA1-Ephrin Type A Receptor 1 ELISA Kit

Human EPHA1-Ephrin Type A Receptor 1 ELISA Kit

409.00583.00

Specification 96T, 48T

SKU: ELK1899 Category: Tag:

Overview of Human EPHA1-Ephrin Type A Receptor 1 ELISA Kit

Product name: Human EPHA1-Ephrin Type A Receptor 1 ELISA Kit
Reactivity: Human
Alternative Names: EPH; EPHT; EPHT1; EPH Receptor A1; EPH tyrosine kinase 1; Erythropoietin-producing hepatoma receptor; Tyrosine-protein kinase receptor EPH
Assay Type: Sandwich
Sensitivity: 0.124 ng/mL
Standard: 20 pg/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5 h
Research Area: Tumor immunity;Neuro science;Developmental science;Hepatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EPHA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EPHA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EPHA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EPHA1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

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