Human F1+2-Prothrombin Fragment 1+2 ELISA Kit
Overview of Human F1+2-Prothrombin Fragment 1+2 ELISA Kit
Product name: |
Human F1+2-Prothrombin Fragment 1+2 ELISA Kit |
Reactivity: | Human |
Alternative Names: | F1+2 |
Assay Type: | sandwich |
Sensitivity: | 0.35 ng/mL |
Standard: | 50 pg/mL |
Detection Range: | 0.79-50 ng/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Hematology |
Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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