Name: Human PKBb-Protein Kinase B Beta ELISA Kit - Gencyst
SKU: ELK2161
Availability: InStock

Human PKBb-Protein Kinase B Beta ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

SKU: ELK2161 Category: ELISA Kit Tag: ELISA Kit

Overview of Human PKBb-Protein Kinase B Beta ELISA Kit

Product name:	Human PKBb-Protein Kinase B Beta ELISA Kit
Reactivity:	Human
Alternative Names:	AKT2; PKBBETA; PRKB-B; RAC-BETA; RAC-Beta Serine/Threonine-Protein Kinase; V-Akt Murine Thymoma Viral Oncogene Homolog 2
Assay Type:	Sandwich
Sensitivity:	0.124 ng/mL
Standard:	20 pg/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5 h
Research Area:	Enzyme & Kinase;Metabolic pathway;Endocrinology;Hormone metabolism;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PKBb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PKBb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PKBb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PKBb in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T, 96T,48T

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