Mouse PTGS2/COX-2-Prostaglandin Endoperoxide Synthase 2 ELISA Kit
Mouse PTGS2/COX-2-Prostaglandin Endoperoxide Synthase 2 ELISA Kit
€318.00 – €455.00
Specification 96T, 48T
Overview of Mouse PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit
Product name: |
Mouse PTGS2/COX-2-Prostaglandin Endoperoxide Synthase 2 ELISA Kit |
Reactivity: | Mouse |
Alternative Names: | PGG/HS; PGHS2; PHS2; HCox2; COX2; Cyclooxygenase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2; PTGS2; Prostaglandin Endoperoxide Synthase 2 |
Assay Type: | Sandwich |
Sensitivity: | 35 pg/mL |
Standard: | 5000 pg/mL |
Detection Range: | 78.13-5000 pg/mL |
Sample Type: | serum, plasma and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Enzyme & Kinase;Tumor immunity;Infection immunity;Reproductive science;Pulmonology;Gastroenterology;Hormone metabolism; |
Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PTGS2/COX-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PTGS2/COX-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PTGS2/COX-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PTGS2/COX-2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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