Human LDH-Lactate Dehydrogenase ELISA Kit

Human LDH-Lactate Dehydrogenase ELISA Kit

409.00583.00

Specification 96T, 48T

SKU: ELK2236 Category: Tag:

Overview of Human LDH-Lactate Dehydrogenase ELISA Kit

Product name:

Human LDH-Lactate Dehydrogenase ELISA Kit

Reactivity: Human
Alternative Names: Lactate Dehydrogenase
Assay Type: Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 pg/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5 h
Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LDH in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

Reviews

There are no reviews yet.

Be the first to review “Human LDH-Lactate Dehydrogenase ELISA Kit”

Your email address will not be published. Required fields are marked *

Filter by price