Mouse CD19-Cluster Of Differentiation 19 ELISA Kit
Overview of Mouse CD19-Cluster Of Differentiation 19 ELISA Kit
Product name: |
Mouse CD19-Cluster Of Differentiation 19 ELISA Kit |
Reactivity: | Mouse |
Alternative Names: | B4; B-lymphocyte surface antigen B4; T-cell surface antigen Leu-12 |
Assay Type: | Sandwich |
Sensitivity: | 0.061 ng/mL |
Standard: | 10 pg/mL |
Detection Range: | 0.16-10 ng/mL |
Sample Type: | Tissue homogenates, cell lysates and other biological fluids. |
Assay Length: | 3.5 h |
Research Area: | Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule; |
Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD19. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD19. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD19, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD19 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
Size | 48T, 96T |
---|
Reviews
There are no reviews yet.