Name: Human F8-Coagulation Factor VIII ELISA Kit - Gencyst
SKU: ELK2249
Availability: InStock

Human F8-Coagulation Factor VIII ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

SKU: ELK2249 Category: ELISA Kit Tag: ELISA Kit

Overview of Human F8-Coagulation Factor VIII ELISA Kit

Product name:	Human F8-Coagulation Factor VIII ELISA Kit
Reactivity:	Human
Alternative Names:	FVIII; AHF; HEMA; F8B; F8C; Antihemophilic Globulin; Hemophilia A; Anti-Hemophilic Factor A; FVIII Procoagulant Component; F8 Protein
Assay Type:	Sandwich
Sensitivity:	30 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	plasma
Assay Length:	3.5 h
Research Area:	Metabolic pathway;Hematology;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F8 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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