Human MYL1-Myosin Light Chain 1, Alkali, Fast Skeletal ELISA Kit
Human MYL1-Myosin Light Chain 1, Alkali, Fast Skeletal ELISA Kit
€409.00 – €583.00
Specification 96T, 48T
Overview of Human MYL1-Myosin Light Chain 1, Alkali, Fast Skeletal ELISA Kit
Product name: |
Human MYL1-Myosin Light Chain 1, Alkali, Fast Skeletal ELISA Kit |
Reactivity: | Human |
Alternative Names: | MLC1; MLC1F; MLC3F; Myosin light chain 1/3, skeletal muscle isoform; Myosin light chain A1/A2 |
Assay Type: | Sandwich |
Sensitivity: | 0.054 ng/mL |
Standard: | 10 pg/mL |
Detection Range: | 0.16-10 ng/mL |
Sample Type: | serum, plasma, tissue homogenates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Infection immunity;Cardiovascular biology; |
Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MYL1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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