Chicken IL2-Interleukin 2 ELISA Kit

Chicken IL2-Interleukin 2 ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2403 Category: Tag:

Overview of Chicken IL2-Interleukin 2 ELISA Kit

Product name: Chicken IL2-Interleukin 2 ELISA Kit
Reactivity: Chicken
Alternative Names: IL-2;TCGF; Lymphokine; T-Cell Growth Factor; Aldesleukin
Assay Type: Sandwich
Sensitivity: 5.9 pg/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Cytokine;Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken IL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken IL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken IL2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Additional information

Size

48T, 96T, 96T,48T

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