Name: Human CRH-Corticotropin Releasing Hormone ELISA Kit - Gencyst
SKU: ELK2411
Availability: InStock

Human CRH-Corticotropin Releasing Hormone ELISA Kit

€409.00 – €583.00

Specification 96T, 48T

Size

Clear

SKU: ELK2411 Category: ELISA Kit Tag: ELISA Kit

Overview of Human CRH-Corticotropin Releasing Hormone ELISA Kit

Product name:	Human CRH-Corticotropin Releasing Hormone ELISA Kit
Reactivity:	Human
Alternative Names:	CRF; Corticotropin Releasing Factor; Corticoliberin
Assay Type:	Competitive Inhibition
Sensitivity:	4.91 pg/mL
Standard:	1000 ng/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	2 h
Research Area:	Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Urology;
Test Principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRH in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T, 96T,48T

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