Mouse OC-Osteocalcin ELISA Kit

Mouse OC-Osteocalcin ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2422 Category: Tag:

Overview of Mouse OC-Osteocalcin ELISA Kit

Product name: Mouse OC-Osteocalcin ELISA Kit
Reactivity: Mouse
Alternative Names: BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
Assay Type: Sandwich
Sensitivity: 0.063 ng/mL
Standard: 10 pg/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OC in the samples is then determined by comparing the OD of the samples to the standard curve.

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Additional information

Size

48T, 96T, 96T,48T

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