Rabbit IFNg-Interferon Gamma ELISA Kit

Rabbit IFNg-Interferon Gamma ELISA Kit

409.00583.00

Specification 96T, 48T

SKU: ELK2424 Category: Tag:

Overview of Rabbit IFNg-Interferon Gamma ELISA Kit

Product name: Rabbit IFNg-Interferon Gamma ELISA Kit
Reactivity: Rabbit
Alternative Names: IFN gamma; IFN-G; IFG; IFI; INFr; IFN; Immune Interferon
Assay Type: Sandwich
Sensitivity: 5.7 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Cytokine;Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit IFNg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit IFNg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit IFNg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit IFNg in the samples is then determined by comparing the OD of the samples to the standard curve.

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Additional information

Size

48T, 96T, 96T,48T

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