Rabbit TF-Tissue Factor ELISA Kit

Rabbit TF-Tissue Factor ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2443 Category: Tag:

Overview of Rabbit TF-Tissue Factor ELISA Kit

Product name: Rabbit TF-Tissue Factor ELISA Kit
Reactivity: Rabbit
Alternative Names: CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III
Assay Type: Sandwich
Sensitivity: 2.63 pg/mL
Standard: 400 pg/mL
Detection Range: 6.25-400 pg/mL
Sample Type: plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Hematology;Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit TF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit TF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit TF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit TF in the samples is then determined by comparing the OD of the samples to the standard curve.

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Additional information

Size

48T, 96T, 96T,48T

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