Human EDN1-Endothelin 1 ELISA Kit

Human EDN1-Endothelin 1 ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2444 Category: Tag:

Overview of Human ET-1-Endothelin1 ELISA Kit

Product name: Human ET-1-Endothelin1 ELISA Kit
Reactivity: Human
Alternative Names: ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1
Assay Type: Sandwich
Sensitivity: 2.71 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Endocrinology;Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Additional information

Size

48T, 96T, 96T,48T

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