Rat G6PD-Glucose 6 Phosphate Dehydrogenase ELISA Kit

Rat G6PD-Glucose 6 Phosphate Dehydrogenase ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2458 Category: Tag:

Overview of Rat G6PD-Glucose 6 Phosphate Dehydrogenase ELISA Kit

Product name: Rat G6PD-Glucose 6 Phosphate Dehydrogenase ELISA Kit
Reactivity: Rat
Alternative Names: Glucose-6-phosphate 1-dehydrogenase
Assay Type: Sandwich
Sensitivity: 0.116 U/L
Standard: 20 U/L
Detection Range: 0.32-20 U/L
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5 h
Research Area: Metabolic pathway;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat G6PD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat G6PD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat G6PD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat G6PD in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

Additional information

Size

48T, 96T, 96T,48T

Reviews

There are no reviews yet.

Be the first to review “Rat G6PD-Glucose 6 Phosphate Dehydrogenase ELISA Kit”

Your email address will not be published. Required fields are marked *

Filter by price