Mouse PTH-Parathyroid Hormone ELISA Kit
Mouse PTH-Parathyroid Hormone ELISA Kit
Specification 96T, 48T
Overview of Mouse I-PTH-IntactParathormone ELISA Kit
| Product name: | Mouse I-PTH-IntactParathormone ELISA Kit |
| Reactivity: | Mouse |
| Alternative Names: | iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone |
| Assay Type: | Sandwich |
| Sensitivity: | 3.94 pg/mL |
| Standard: | 800 pg/mL |
| Detection Range: | 12.5-800 pg/mL |
| Sample Type: | serum, plasma and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Metabolic pathway;Endocrinology;Hormone metabolism; |
| Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
| Size | 96T,48T |
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